Alma Mater Studiorum - University of Bologna

The University of Bologna has ancient origins: founded in 1088, it is considered the oldest University in the western world. It has over 80,000 students and 210 degree programs, 59 of which are international degree programs, in its 5 Campuses.

Via Zamboni, 33, 40126 Bologna BO, Italy

SILICA PARTICLES AS LUMINESCENT MARKERS ALTERNATIVE TO THE TANDEM-DYES

managed by Alma Mater Studiorum UNIBO

TECHNOLOGY DESCRIPTION

Using some silica particles in combination with a selection of dyes provided with particular spectral characteristics, it is possible to obtain systems with high Stokes shift, even using a single wavelength of excitation and in which the problem of the car off of the dyes is avoided or substantially reduced. At the same time you get a dye which has a fluorescence emission at a longer wavelength, although the excitation is made at a shorter wavelength. The object of the present invention is thus a silica particle comprising a micelle having a substantially hydrophilic outer shell and a central part substantially hydrophobic; a core, which is located in the central portion of the micelle and includes a lattice of silicate; a plurality of molecules, which comprises at least one surfactant molecule functionalized.

BENEFITS

N/A

LIMITATIONS

N/A

APPLICATIONS

The method is proposed as suitable to develop luminescent markers in which there is a high separation between the wavelength of excitation and the emission. Among the immediate applications there are those related to flow cytometry. In addition to these advantages, we can mention:
Sensitization very high and very low residual emission of the acceptor end of the different donors, when the particle contains more than two dyes;
Very large Stokes shifts, up to 700-800 nm;
Very high brightness, even exciting the donor at higher energy (largest donor in blue);
Residual intensity altogether negligible for all dyes, except for the last acceptor.

Readiness Level (TRL)

4
technology validated in lab(The pie chart refers to the entire portfolio contained in the database of the helpdesk)

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STATUS

Current status

JP Application

AVAILABILITY

Available for

Licensing or Assignment

INVENTOR / TEAM

Luca Prodi, Sara Bonacchi, Marco Montalti, Nelsi Zaccheroni, Riccardo Juris, Damiano Genovese, Enrico Rampazzo

GENERAL ENQUIRIES
LICENSING REQUEST

Case manager: Maria Grazia Fumo

EU-Japan Centre
European CommissionMeti