Centro di Riferimento Oncologico (IRCCS CRO AVIANO) - National Cancer Institute

CRO is an Italian public institute founded in 1984 and is committed to pursue excellence in all aspects of clinical oncology and management of health services. CRO is also an excellence in translational and clinical research activity for the advancement of scientific knowledge.

Via Franco Gallini, 2, 33081 Aviano PN, Italia

Twist gain-of-function and loss-of-function cell models

managed by IRCCS CRO AVIANO

TECHNOLOGY DESCRIPTION

Twist GAIN-of-function and LOSS-of-function cell models were developed and used for preclinical p53:MDM2 inhibitors evaluation, preliminary demonstrating that the expression of Twist hampers their efficacy.
These findings support patient stratification on the basis of twist expression for treatment of a large fraction of sarcomas but also of a fraction of carcinomas. (Piccinin et al., Cancer Cell, 22, 404-415, Sept 11 2012).
Developmental stage:
- 3 different cell lines were consistently characterized.
- 2 more cell lines are producing promising results.
- 6 different drugs have already been tested including nutlin3a - AMG 232 - RG7112 - SAR405838. More than 100 cell vitality tests have been performed.

BENEFITS

Standard p53 wild-type cell models need to be engineered for Twist expression to be used for iMDM2 testing.

LIMITATIONS

The main limitation is that these models are designed for screening purposes therefore they can not reflect actual single patient response.

APPLICATIONS

The main application is to evaluate activity of p53:MDM2 inhibitors in preclinical phase.
These cell models can be used also as research tools to explore p53 inactivation mechanisms in TP53 wild type tumors, and to study MDM2i resistance mechanisms.

Readiness Level (TRL)

4
technology validated in lab(The pie chart refers to the entire portfolio contained in the database of the helpdesk)

Technology Readiness Levels (TRL) are a method of estimating technology maturity of Critical Technology Elements (CTE) of a program during the acquisition process. The use of TRLs enables consistent, uniform, discussions of technical maturity across different types of technology.

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STATUS

Current status

Currently the knowhow related to the technology is secret. The best IPR protection strategy is under evaluation.

AVAILABILITY

Available for

Licensing

INVENTOR / TEAM

Roberta Maestro, Sara Piccinin

EU-Japan Centre
European CommissionMeti